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主营:体外转录、基因克隆(BAC克隆试剂盒)、感受态细胞(TG1噬菌体文库构建感受态)、体外转录、转座子突变、蛋白表达
℡ 4000-520-616
℡ 4000-520-616
Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/60052-2/24 rxns (DUOs)
产品编号:60052-2
市  场 价:¥5580.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$279.00
品      牌: Lucigen
公      司:Lucigen
公司分类:
Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/60052-2/24 rxns (DUOs)
商品介绍
  • Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)
  • Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.
  • Availableinarangeofhightransformationefficiencies(5×109to4×1010cfu/µg).
  • Convenientpackagingoptions.

Lookingforchemicallycompetentversion?

Free Sample AvailableFreeSampleAvailable

 

Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliABIlity(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.

Choiceofstrain:

  • E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
  • E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.

Choiceofefficiency:

  • E.cloni10GSUPREMEElectrocompetentCells>4×1010cfu/µgpUCDNA
    SUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossIBLe.
  • E.cloni10G&10GF´ELITEElectrocompetentCells>2×1010cfu/µgpUCDNA
    ELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice.
  • E.cloni10GCLASSICElectrocompetentCells>5×109cfu/µgpUCDNA
    CLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.

Custom CellsCustomCompetentCells

BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.


Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleADIngsupplier(Figures1and2)

Recombinant Yields

 

Transformation Efficiency

Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16).

 

Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells)

Table1.E.cloniCompetentCellsforCloningandLibraryConstruction


E.cloniCellLines

TransformationEfficiency
(cfu/µgpUCDNA)

CloningMethylatedDNA

BAC,CosmidCloning

Blue/WhiteScreening

10GSUPREMEElectrocompetent

>4×1010

YES

YES

YES
withoutIPTGinduction

10GELITEElectrocompetent

>2×1010

YES

NO

YES
withoutIPTGinduction

10GCLASSICElectrocompetent

>5×109

YES

NO

YES
withoutIPTGinduction

10GF´ELITEElectrocompetent

>2×1010

YES

NO

YES
IPTGinductionrequired

Efficiencies

  • E.cloni 10GSUPREMEElectrocompetentCells > 4×1010 cfu/µgpUCDNA
  • E.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 1010 cfu/µgpUCDNA
  • E.cloni 10GCLASSICElectrocompetentCells > 5 × 109 cfu/µgpUCDNA 

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

品牌介绍

Lucigen


美国Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


Lucigen Corporation is working to improve people’s lives by providing quality products and services to life science and healthcare professionals. Founded in Wisconsin in 1998, Lucigen offers products and services that enable life-science professionals to perform their research and testing more efficiently and effectively. The company has grown to manufacture and sell over 325 biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme evolution, protein expression, cloning, competent cells, next gen sequencing, and molecular diagnostics.


产品列表:



No.

品牌

货号

名称

规格

1

lucigen

40041-2

CloneSmart HCAmp Blunt Cloning Kit

20 rxns

2

lucigen

40300-2

CloneSmart LCAmp Blunt Cloning Kit

 20 rxns

3

lucigen

RG90910K

RiboGuard™ RNase Inhibitor

10,000 Units

4

lucigen

AS3107

AmpliScribe™ T7 High Yield Transcription Kit

50 Reactions

5

lucigen

ASB71110

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

10 Reactions


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