FreeSampleAvailable
MC1061F-ElectrocompetentCells.Anon-ambersuppressorstrain,identicaltoSS320exceptthatitlackstheF′episome.Highlyefficient(≥4×1010cfu/µg)electrocompetentcellsforgeneralcloningorPhageDisplay.NotethatasanF-strain,itcannotbeusedforre-infectionbyfilamentousphage. Thisstrainnotincludedinthesamplekit.
Figure1.TransformationefficiencyofLucigen'selectrocompetentbacterialcellsforphagedisplaycomparedtocompetitor'sspecification. |
Table1.CompetentCellsforPhageDisplaylibraries
CellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
TG1Electrocompetent | >4×1010 | YES | NO | YES |
SS320(MC1061F')Electrocompetent | >4×1010 | YES | NO | YES |
MC1061F-Electrocompetent | >4×1010 | YES | NO | YES |
ER2738Electrocompetent | >2×1010 | YES | NO | YES |
No. | 品牌 | 货号 | 名称 | 规格 |
1 | lucigen | 40041-2 | CloneSmart HCAmp Blunt Cloning Kit | 20 rxns |
2 | lucigen | 40300-2 | CloneSmart LCAmp Blunt Cloning Kit | 20 rxns |
3 | lucigen | RG90910K | RiboGuard™ RNase Inhibitor | 10,000 Units |
4 | lucigen | AS3107 | AmpliScribe™ T7 High Yield Transcription Kit | 50 Reactions |
5 | lucigen | ASB71110 | AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit | 10 Reactions |