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当前位置: 首页 > 产品中心 > > Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351025/25 rxn

Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351025/25 rxn

Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351025/25 rxn
  • Lucigen/Sygnis TruePrime™ Single Cell WGA Kit version 2.0/SYG351025/25 rxn
商品介绍

Singlecellwholegenomeamplification(WGA)withlessbiasmakingthiskitideallysuitedfornextgenerationsequencingapplications.

  • Highsensitivity:Typicalyieldisgreaterthan5µgwhenstartingwithasinglemammaliancell.
  • Reducedamplificationbiasandnoprimerartefacts:Primer-freeamplificationmethodutilizesacombinationofTthPrimPolPrimase(tosynthesizeprimers)andPhi29DNApolymerase.
  • Wellsuitedfornextgenerationsequencing:TestedinIlluminaandIonTorrentNGSworkflows.
  • Easyandreliable
  • InsensitivetoexternalDNAcontamination

  • HowdoesTruePrime™Technologywork?
  • Wholegenomeamplificationfrom5cells
  • Absenceofprimerartefacts
  • InsensitivitytoexternalDNAcontaminations
  • Excellentgenomecoverage

HowdoesTruePrimetechnologywork?

Single Cell Whole Genome Amplification How It Works

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Wholegenomeamplificationfrom5cells

Whole Genome Amplification from 5 cells

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Absenceofprimerartefacts

TruePRime Single Cell Whole Genome Amplification has absence of artefacts

1pgofhumangenomicDNA(~1/6ofthecontentofonehuman/mammaliancell)hasbeenamplifiedusingeitherTruePrime™(TthPrimPol-basedMDA)orrandomprimedMDAreactions.Randomprimedreactionscontain20%ofsequencesthatcannotbemappedtoanyorganisminsequencedatabases.

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InsensitivitytoexternalDNAcontaminations

Duetothehighsensitivityofsinglecellwholegenomeamplificationtechniquesthereisaconsiderabledangerofcontaminatingreactionswithnon-targetderivedDNA.Wethereforerecommendtotakeextremecarewhensettingupsinglecellamplificationreactionsaslaidoutinthehandbook.However,TruePrime™usershaveauniqueadvantagehere:TruePrime™doesnotacceptnon-denaturedDNAstrandsastemplateasreADIlyasrandomprimedMDAreactions.Therefore,contaminationscominginfromtheenvironmentetc.afterthedenaturationstepwillnothaveastronginfluenceonthecompositionofyourreactionoutput.WehaveshownthisbehaviourinanexperimentwherewesimulateanexternalDNAcontaminationbydeliberatelyaddinginyeastDNAtoadenaturedhumanDNAtemplate.YeastDNAisinathousand-foldexcessoverthetargetDNA(1pghumangenomicDNA):

TruePrime Single Cell WGA Insensitive to External DNA Contamination

WhereaswithTruePrime™thevastmajorityofthesequencesobtainedaretarget-derived,randomprimedMDAshowsamajorityofcontaminant-derivedsequences.

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Excellentgenomecoverage

TruePrime Coverage Yeast Chromosome 7

WehaveamplifiedandsequencedtheyeastgenomeusingTruePrime™andIlluminatechnology.Using2.5millionreadpairswecover99.4%ofthetotalyeastgenome.Shownisthecoveragewithnarrowwidthofthedistributioncurve.

Genome coverage of Yeast

Acoveragegraphofyeastchromosome7exemplifiesthemoreevencoverageobtainedbyTruePrime™MDA(middlebluelinerepresentsmeancoverage).

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ORDERINFORMATION

EachTruePrime™SingleCellWGAKitversion2.0contains:BufferL1,DTT,BufferN,ReactionBuffer,dNTPs,Water,Enzyme1,Enzyme2,Control1,andControl2.ThecompletemanualisavailableundertheManualtab.LucigenisanauthorizeddistributorofSygnisproductsintheUS.

TruePrime™SingleCellWGAversion2.0kitisintendedformolecularBIOLOGyuseonlyandinvitrouseonly.Thisproductisnotintendedfordiagnosis,preventionortreatmentofadiseaseinhumanbeingsoranimals.
品牌介绍

Lucigen


美国Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品,凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。


Lucigen Corporation is working to improve people’s lives by providing quality products and services to life science and healthcare professionals. Founded in Wisconsin in 1998, Lucigen offers products and services that enable life-science professionals to perform their research and testing more efficiently and effectively. The company has grown to manufacture and sell over 325 biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme evolution, protein expression, cloning, competent cells, next gen sequencing, and molecular diagnostics.


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