• Only source for recombinant T4 DNA Polymerase, Exonuclease Minus.
  • Potential applications include enhancing DNA sequencing reads.

T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer. Additionally, the enzyme lacks 3’→5’ exonuclease activity.

Source: Purified from a strain of E. coli that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.

Applications:

  • DNA Sequencing reaction.(1)

Heat inactivation: 70°C for 15 minutes.

Storage: Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

  • May increase the DNA sequencing read-lengths.

References

  • Nancy G. Nossal (1998) Genetics 148: 1535-1538.

    • ORDER INFORMATION

    Recombinant T4 DNA Polymerase, Exonuclease Minus is supplied at a concentration of 3,000U/ml in a storage buffer of 50% glycerol, 100 mM KPO4 (pH 6.5) and 1 mM DTT. Also supplied is 10X DNA Polymerase Buffer A composed of 100 mM Tris-HCl (pH7.9), 100 mM MgCl2, 500 mM NaCl and 10 mM DTT.