• OnlysourceforrecombinantT4DNAPolymerase,ExonucleaseMinus.
  • PotentialapplicationsincludeenhancingDNAsequencingreads.

T4DNAPolymerase,ExonucleaseMinusisaDNA-dependentDNApolymerasewhichcatalyzesthe5’→3’synthesisofDNAandrequirestemplateandprimer. Additionally,theenzymelacks3’→5’exonucleaseactivity.

Source:
PurifiedfromastrainofE.colithatcarriesaT4DNAPolymerase,ExonucleaseMinusexpressionplasmid.

Applications:

  • DNASequencingreaction.(1)

Heatinactivation:70°Cfor15minutes.

Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.

AdditionalInformation:

  • MayincreasetheDNAsequencingread-lengths.

References

  1. NancyG.Nossal(1998)Genetics148:1535-1538.

ORDERINFORMATION

RecombinantT4DNAPolymerase,ExonucleaseMinusissuppliedataconcentrationof3,000U/mlinastoragebufferof50%glycerol,100mMKPO4(pH6.5)and1mMDTT.Alsosuppliedis10XDNAPolymeraseBufferAcomposedof100mMTris-HCl(pH7.9),100mMMgCl2,500mMNaCland10mMDTT.