Magnesium-dependentdigestionofsingle-strandedDNAina5´to3´direction

  • Epicentre ProductSpecific:DigestsssDNAinthe5'to3direction
  • HeatInactivated:Heatat65°Cfor20minutestokillenzymeactivity
  • Flexible:UsetoremoveprimersfromcompletedPCRortodegradesingle-strandedlinearDNAindsDNAandplasmidpreps

RecJExonuclease,derivedfromE.coli,catalyzesdegradationofssDNAina5´to3´direction.ItsactivityisdependentonMg2+.RecJExonucleasecanbeheat-inactivatedbyincubationat65°Cfor20minutes.

Applications

  • RemovalofprimersfromcompletedPCR.
  • Degradationofsingle-strandedlinearDNAindsDNAandplasmidpreps.

UnitDefinition:OneunitofRecJExonucleasecatalyzesthereleaseof1nmolofacid-solublenucleotidesfromactivatedsingle-strandedcalfthymusDNAin30minutesat37°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),0.1mMEDTA,1mMDTT,0.1MNaCl,and0.1%Triton®X-100.

10XReactionBuffer:330mMTris-acetate(pH7.5),660mMpotassiumacetate,100mMmagnesium,acetate,and5.0mMDTT.

QualityControl:RecJExonucleaseisfreeofdetectableRNaseandcontaminatingDNAendonucleaseactivities.

ORDERINFORMATION

Includes10XReactionBuffer.